Plasmid_Backbone
Part:BBa_K1677202:Design
Designed by: Mackenzie Labine-Romain Group: iGEM15_BABS_UNSW_Australia (2015-09-15)
psbA2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal EcoRI site found at 4865
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 28
Illegal NotI site found at 4871 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal EcoRI site found at 4865
Illegal BglII site found at 22
Illegal BamHI site found at 839
Illegal XhoI site found at 4201 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4865
Illegal suffix found at 2
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4865
Plasmid lacks a suffix.
Illegal EcoRI site found at 354
Illegal EcoRI site found at 4234
Illegal XbaI site found at 987
Illegal XbaI site found at 4880
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 3864 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2688
Illegal SapI site found at 1605
Design Notes
Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.
Source
Addgene registry accession #52307