Plasmid_Backbone

Part:BBa_K1677202:Design

Designed by: Mackenzie Labine-Romain   Group: iGEM15_BABS_UNSW_Australia   (2015-09-15)


psbA2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 354
    Illegal EcoRI site found at 4234
    Illegal XbaI site found at 987
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 354
    Illegal EcoRI site found at 4234
    Illegal EcoRI site found at 4865
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 28
    Illegal NotI site found at 4871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 354
    Illegal EcoRI site found at 4234
    Illegal EcoRI site found at 4865
    Illegal BglII site found at 22
    Illegal BamHI site found at 839
    Illegal XhoI site found at 4201
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4865
    Illegal suffix found at 2
    Illegal EcoRI site found at 354
    Illegal EcoRI site found at 4234
    Illegal XbaI site found at 987
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4865
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 354
    Illegal EcoRI site found at 4234
    Illegal XbaI site found at 987
    Illegal XbaI site found at 4880
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 3864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2688
    Illegal SapI site found at 1605


Design Notes

Primers containing biobrick prefix and suffix as well and other described parts were used to make this backbone RFC10 compatible.



Source

Addgene registry accession #52307

References